The existing passenger flow counting sensors with PolyVinyliDene Fluoride (PVDF) piezoelectric material exist the lack of accuracy caused by erroneous counting and missing counting, which has characteristics of low cost and resistance to wear and tear. In order to solve the problem, a passenger counting system based on PVDF gait intelligent detection technology was proposed. The ANSYS software was applied to carry out stress analysis of passengers' gait stepping on and off the bus and observe the distribution of the PVDF piezoelectric signal. The multi-input signal conditioning circuit was designed to acquire multi-channel plantar signal. Combined with signal processing algorithm, the sensor mechanical structure and people-counting system on buses were introduced by Laboratory Virtual Instrument Engineering Workbench (LabVIEW).The experimental results indicate that the proposed system improves the precision in comparison with the existing PVDF passenger flow counting sensors, reduces the cost in comparison with video image counting and human body infrared detection technology, and the average counting error is 5.3%.The proposed system has high practicality and can be widely used in Chinese public transport buses.